别名 氨基丁三醇 | Trizma 碱 | 三羟甲基氨基甲烷 | Tris 碱 | 2-氨基-2-(羟甲基)-1,3-丙二醇 | 三羟甲基氨基甲烷
英文别名 2-Amino-2-(hydroxymethyl)-1,3-propanediol | Trimethylolaminomethane | THAM
规格或纯度 ≥99%(titration)
英文名称 Tris(hydroxymethyl)aminomethane (Tris base)
应用 Tris base has been used:In the dissection medium and culture medium of Hippocampal organotypic tissue cultures；As a component of lysis buffer before immunoprecipitation and immunoblotting；As a component of harvest buffer, lysis buffer, ChIP dilution buffer, and wash buffer in chromatin immunoprecipitation method for studying protein-DNA binding；As a component of mixed buffer formulations；As a component of NuPAGE loading buffer for SDS-PAGE and as a component of transfer buffer for Western blotting；Trizma is used in the formulation of buffer solutions in the pH range between 7.5 and 8.5. Tris buffer solutions are widely used in cell and molecular biology for processes such as protein and nucleic acid extraction and purification. Trizma based buffers are also in column chromatography and in gel electrophoresis. Trizma base is used as a general reagent for the preparation of all types of Tris buffers.
储存条件 室温
运输条件 常规运输
纯度 ≥99%(titration)