规格或纯度:BioReagent
英文名称:Mouse IL-6 ELISA Kit
应用:ELISA
样品类型:体液, 组织匀浆, 细胞培养上清, 血浆, 血清
检测范围:7.8 pg/mL~500 pg/mL
检测方法:Sandwich ELISA
检测物种:小鼠
特异性:可检测样本中的小鼠白细胞介素6，且与其它类似物无明显交叉反应
检测仪器:酶标仪
检测波长:450nm
产品介绍: 本试剂盒采用双抗体夹心ELISA法检测样品中小鼠白细胞介素6的浓度。小鼠白细胞介素6捕获抗体已经预包被于酶标板上，当加入样品或标准品时，其中的小鼠白细胞介素6会与捕获抗体结合，而其它游离成分则会通过洗涤被除去。接着，再加入生物素标记的小鼠白细胞介素6抗体后，抗小鼠白细胞介素6抗体与小鼠白细胞介素6接合，形成夹心的免疫复合物，其它游离成分则通过洗涤被除去。随后加入酶复合物，生物素与酶复合物特异性结合，这样酶复合物上的HRP就与夹心的免疫复合物连接起来，而其它游离成分则通过洗涤被除去。最后加入显色剂，若样品中存在小鼠白细胞介素6，则会形成免疫复合物，其上连接的HRP会催化无色的显色剂氧化生成蓝色物质，而后加入终止液，最终产物呈黄色。通过酶标仪检测，读取450nm处的OD值，小鼠白细胞介素6浓度与OD450值之间呈正比，通过检测标准品绘制标准曲线，对照未知样品中OD值，即可计算出样品中小鼠白细胞介素6的浓度。
This kit employs a sandwich enzyme-linked immunosorbent assay (ELISA) for the quantitative detection of Mouse IL-6 concentration in samples. The Mouse IL-6 capture antibody is pre-coated onto the microtiter plate. Upon addition of samples or standards, Mouse IL-6 present therein binds to the immobilized capture antibody, while other unbound components are removed through washing. Subsequently, a biotin-conjugated detection antibody specific for Mouse IL-6 is added. This antibody binds to the captured Mouse IL-6, forming a "sandwich" immunocomplex. Any excess, unbound material is again removed by washing. Next, an enzyme conjugate (typically Streptavidin-Horseradish Peroxidase, SA-HRP) is added. The biotin on the detection antibody and streptavidin on the enzyme conjugate exhibit high-affinity binding, thereby linking the HRP enzyme to the immunocomplex. Following another wash step to remove unbound conjugate, a colorimetric substrate (e.g., TMB) is added. If Mouse IL-6 is present in the sample, the HRP linked to the complex catalyzes the oxidation of the colorless substrate, yielding a blue product. The reaction is then stopped by adding a stop solution (usually an acid), which changes the color from blue to yellow. The optical density (OD) of each well is measured at 450 nm using a microplate reader. The concentration ofMouse IL-6 is directly proportional to the OD450 value. A standard curve is generated by assaying known concentrations ofMouse IL-6, and the concentration of Mouse IL-6 in unknown samples is interpolated from this curve based on their OD values.